International Research Staff Exchange Scheme (IRSES)


MC-IRSES Nr.316177, BIOSENSORS-AGRICULT. 292 600 € (implementation  01.09.2012.-31.08.2016.)

The project  BIOSENSORS-AGRICULT – P7-2012-IRSES, GA 318529, Start date 01.09.2012. Marie Curie Actions- International Research Staff Exchange Scheme (IRSES)

Project presentation – (click) IRSES 291311_FP7 BIOSENSORS AGRICULT



2. List of participants (beneficiaries and participant /organisations)

Participant   Number

Participant   name

Participantshort   name


1 Beneficiary   1 Coordinator,    University of LatviaAssociation   FOTONIKA-LV,(



2 Beneficiary   2 University of  Linkoping, Department of   Physics, Chemistry and Biology( )



3 Beneficiary 3 CENTRE NATIONAL   DE LA RECHERCHE SCIENTIFIQUE European Institutes of Membranes (



4 Participant 4 NATIONAL UNIVERSITY OF LIFE AND   ENVIRONMENTAL SCIENCES OF UKRAINE,   Department of Molecular Biology Microbiology and Biosecurity (



5 Participant 5


Faculty of Physics, Faculty of Biology and   Faculty of Chemistry, (,



6 Participant 6 Institute of Biophysics and Cell   Engineering, National Academy of Sciences of Belarus,(



4.1.3. The state-of-the-art and general scientific content of the foreseen research.

Metal oxides are well known materials for sensor technologies[1]. They have a number of advantages such as stability in aggressive environment, well studied properties and many technological routes to deposit[2]. During last decade the attention has been paid to metal oxide nanostructures[3] because nanosystems have the smallest dimension structures that can be used for efficient transport of electrons and are thus critical to the function and integration of these nanoscale devices. Because of their high surface-to-volume ratio and tunable electron transport properties through quantum confinement effect, their electrical, optical and sensitive properties are strongly sensitive to minor perturbations[4].

As a result of quantum confinement effect, new optical properties emerge in metal oxide nanostructures, e.g. photoluminescence. It was found, that at room temperature photoluminescence in ZnO, TiO2, Al2O3 and SiO2 nanostructures resulted from the decrease of crystalline size[5],[6],[7]. The nature of photoluminescence in metal oxide nanostructures was explained by excitons and deep biographic defect levels[8].

Metal oxides are good candidates for templates in biosensitive layers. They show good affinity to biological molecules. Among a number of metal oxides, TiO2, Al2O2, ZnO, SnO2 and SiO2 are widely used for sensor and biosensor application. This peculiarity was widely used in amperometric biosensors to detect glucose[9]. However, other optical properties such as absorption and photoluminescence are not sufficiently often used in biosensor devices.

Optical fibers are novel technologies for different applications. Because of their dimensions and flexibility they can be exposed in various places. Optical fibers are good transducers for sensor applications. They ensure high precision measurements and allow using different measurement methods such as absorption, reflectance, transmittance and photoluminescence[10]. It was shown that metal oxides can be deposited on the end surface of optical fibers and used for detection of low concentrations of ammonia in water solutions[11]. Complex characterisation of the metal oxide sensitive layer was performed and sensor characteristics were investigated. However, further investigations to increase sensor selectivity and to study this system for biosensor applications were not done.

On-chip technology is the one which is widely used in medical applications (glucometers and so on). It is based on vibration methods with piezoelectric biosensitive templates and fluorescent methods[12]. The advantage of such biosensors is possibility to vary biosensititve layers and even to create arrays of biosensors on one chip to do complex analysis[13]

Traditionally, biosensors are applied in the domain of medicine[14] and in various other domains like environment, defence bioprocessing, food technology etc.[15]. Agriculture has needs and applications for biosensors detection[16] . It is important to control soil content, to control food quality and to prevent animal and plant diseases.

Early, the immune biosensor based on the SPR and foreseen for the bovine leucosis diagnostics have been developed[17]. This biosensor may provide direct (without any additional reagents), rapid (during 3-5 min), cheap (less 1$) detection even without blood samples and using a drop of milk. The analysis of 20 of standard positive and negative antiserums by three different methods (RID, ELISA-method, PLR and SPR based immune biosensor) has shown a good correlation in the obtained results[18]. Two and one differences were checked between data of the immune biosensor and the ELISA-method and polymerase chain reaction, respectively.

In [19] the studies of silica nanofibers hydrogels for biosensors applications have been reported. Photoluminescence (PL) spectra of SiO2 hydrogels with immobilized antigens (Ag) and antibodies (Ab) in the range were studied in the region 370-620 nm. Experimental dependence of PL properties of SiO2 hydrogels with immobilized Ag on Ab concentration was studied. This method showed better results for leucosis detection than SPR method.

Therefore, from the findings discussed above conclusion can be made that optical fiber biosensors with sensitive layer of nanostructured metal oxide and on-chip biosensors can be good candidates for application in the agriculture sector to implement different tasks from soil monitoring to food quality control.

[1] V. E. Bochenkov, G. B. Sergeev, Sensitivity, Selectivity and Stability of Gas-Sensitive Metal Oxide Nanostructures, Volume 3: Pages 31-52, Metal Oxide Nanostructures and Their Applications, Copyright © 2010 by American Scientific Publishers, Edited by Ahmad Umar and Yoon-Bong Hahn, ISBN: 1-58883-176-0

[2] Pratima R. Solanki, Ajeet Kaushik, Ved V. Agrawal and Bansi D. Malhotr, Nanostructured metal oxide-based biosensors, NPG Asia Mater. 3(1), 17–24 (2011),  doi:10.1038/asiamat.2010.137

[3] Umasankar Yogeswaran and Shen-Ming Chen, A Review on the Electrochemical Sensors and Biosensors Composed of Nanowires as Sensing Material, Sensors, 2008, 8, 290-313

[4]Hernandez-Velez, M. Nanowires and 1D arrays fabrication: An overview. Thin Solid Films. 2006, 495, 51-63; DOI 10.1016/j.tsf.2005.08.331.

[5] P. Dennis Christy, N. S Nirmala Jothi, N. Melikechi, and P. Sagayaraj,  Synthesis, structural and optical properties of well dispersed anatase TiO2 nanoparticles by non-hydrothermal method, Cryst. Res. Technol. 44, No. 5, 484 – 488 (2009).

[7] J. del-Castillo , AC Yanesl, J. Méndez-Ramos , VD. Rodríguez  Luminescence of nanostructured SnO2-SiO2 glass-ceramics prepared by sol-gel method, J Nanosci Nanotechnol. 2008, 8(4), 2143-2146

[8] Ping Wu, Qiang Li, Xingquan Zou, Wende Cheng, Danli Zhang, Chuanxi Zhao, Lingfei Chi, Tan Xiao Correlation between photoluminescence and oxygen vacancies in In2O3, SnO2 and ZnO metal oxide nanostructures, Journal of Physics: Conference Series 188 (2009) 012054 doi:10.1088/1742-6596/188/1/012054

[9] Tao Kong, Yang Chen, Yiping Ye, Kun Zhang, ZhenxingWang, XiaopingWang. An amperometric glucose biosensor based on the immobilization of glucose oxidase on the ZnO nanotubes, Sensors and Actuators B, 2001, 138, 344–350.

[10] María Espinosa Bosch, Antonio Jesús Ruiz Sánchez , Fuensanta Sánchez Rojas and Catalina Bosch Ojeda Recent Development in Optical Fiber Biosensors, Sensors, 2007, 7, 797-859

[11] M. Pisco, M. Consales, R. Viter, V. Smyntyna, S. Campopiano, M. Giordano, A. Cusano, A.Cutolo Novel SnO2 based optical sensor for detection of low ammonia concentrations in water at room temperatures, Intern. Sc. J. Semiconductor Physics, Quantum Electronics and Optoelectronics.- 2005.- Vol. 8.- p.95-99.

[12] Geschke, Klank & Telleman, eds.: Microsystem Engineering of Lab-on-a-chip Devices, 1st ed, John Wiley & Sons.  ISBN 3-527-30733-8

[13] Yehya H. Ghallab, Wael Badawy (2010). Lab-on-a-chip: Techniques, Circuits, and Biomedical Applications. Artech House. ISBN 1596934182, 9781596934184. 220 

[14] A. Kishen, M.S. John, C.S. Lim, A. Asundi. A fiber optic biosensor (FOBS) to monitor mutans streptococci in human saliva, Biosensors and Bioelectronics 18, (2003), 1371-1378

[15]  María Espinosa Bosch, Antonio Jesús Ruiz Sánchez, Fuensanta Sánchez Rojas, Catalina Bosch Ojeda. Recent  Development in Optical Fiber Biosensors,  Sensors 2007, 7, 797-859

[16] J.S Rana, Jyoti Jindal, Vikas Beniwal, Vinod Chhokar Utility Biosensors for applications in Agriculture – A Review,

Journal of American Science, 2010, 6(9), 353-375

[17] Starodub N. F., Arthjuch V. P., Pirogova L.V., Nagajeva L. I., Dobrosol G. I., Pavlenko M., Grotevich VExpress diagnostics of bovine leucosis on the basis of biosensor analysis. Vet. Med., 2001, N11, p. 26-27.

[18]  Starodub N. F., Starodub V. N. Infectious bovine leucosis and its diagnostics, Biopolymers and Cell, 2003, 19, 307-316

[19] R. Viter, N. Starodub, V. Smyntyna, A.Tereschenko, A. Kusevitch, J. Sitnik, J. Buk, J. Macak, Immune Biosensor Based on Silica Nanotube Hydrogels for Rapid Biochemical Diagnostics of Bovine Retroviral Leukemia, Procedia Engineering, 25, 948-951 (2011).